Streptococcus uberis on SELMA and SELMA Plus plates – new images

Streptococcus uberis on SELMA and SELMA Plus plates

Streptococcus uberis cultivated on A, SELMA and B, SELMA Plus plates. Image: Karl-Erik Johansson (SLU), Fereshteh Banihashem (SVA) & Helén Mönnig (SVA).

In Sweden, the proportion of S. uberis induced acute clinical mastitis constituted 11% over the past 10 years. SELMA and SELMA Plus plates are used for cultivation of milk samples from suspected mastitis cases. It is often possible to make a preliminary diagnosis from the results. Note that S. uberis is only growing in the sector with bovine blood agar with esculine.

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Bordetella bronchiseptica – new images

 

Bordetella bronchiseptica

Gram staining of Bordetella bronchiseptica at two different degrees of magnification.

Bordetella bronchiseptica is an impotant bacterium in veterinary medicine. This bacterium cause primary and secondary airway infections in dogs and pigs. The old images on the bacterial page of B. bronchiseptica have been replaced by images of higher quality and some new ones have also been added. Colonies of B. bronchiseptica on blood agar, purple agar with lactose, MacConkey agar and Smith & Baskerville agar are now included on the bacterial page of B. bronchiseptica as well as Gram staining images.

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Comments on VetBactLab in Swedish

Tack vare uppmärksamma veterinärstudenter på baktkursen har vi nu kunnat rätta till ett par felaktigheter och en tvetydighet förklaras i sista punkten nedan:

  •  I verktyget Kolhydratkombination 3 för fall 1 var plus (+) och minus (-) omkastade på kontrollrören. Det är rättat nu.
  • Om man använde webbläsaren Internet Explorer, så visades inte kontaktformuläret när man ville lämna en kommentar efter ett genomgånget fall. Nu öppnas formuläret i en ny flik i stället för ett pop-up fönster och det verkar fungera.
  • När man väljer verktyget Kolhydratkombination 4 [Carb. comb. 4 (Samonella)], kan man se (om man klickar på verktygsnamnet) att detta verktyg omfattar 3 kolhydrater, som också förekommer i de andra kombinationerna, samt ytterligare en kolhydrat, nämligen sorbosfosfat. Observera att sorbosfosfat inte är samma som sorbitol och att information om hur Salmonella spp. fermenterar sorbosfosfat hittar man i en fotnot till kolhydrattabellen på bakteriesidan för S. enterica susp. enterica och inte i själva kolhydrattabellen. Nu är denna information också inskriven i verktygsbeskrivningen i VetBactLab.
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Welcome to the Bacteriology course, 2012

Kaggen giving a lecture on VetBact.
Kaggen giving a lecture on VetBact, 25 September 2012.   Photo: Staffan Tamm/VetBact.org

On September 24 the bac­­te­­rio­­logy course for the veterinary students started. We hope that you will find Vet­Bact useful during your studies and we appre­ciate all kinds of feed-back, which will help us to improve VetBact and its three modules: VetBactQuiz, VetBactBlog and VetBactLab.

Feed-back can be given in different ways:

  1. By leaving a comment on this post (in English or Swedish) in the VetBactBlog. The comment will be published on the Blog if it is relevant.
  2. By using the contact form, which can be reached from most pages of VetBact (particularly useful if you want to comment or ask a question about a certain bacterial species).
  3. By leaving a comment on the form, which can be opened when you have finished a case in VetBactLab (particularly useful if you want to comment on the in silico cases).

You may of course also ask questions concerning bacteriology by any of the above ways.

The VetBactRef group

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Salmonella enterica subsp. enterica and Escherichia coli – new images

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Salmonella enterica subsp. enterica cultivated on bovine blood agar.

Old VetBact images of Salmonella enterica subsp. enterica and Escherichia coli (cultivated on bovine blood agar) have now been replaced with images of improved quality. Images of S. enterica and E. coli  cultivated on purple agar and on special media (BG agar, MSRV agar and XLD agar) have also been added to the bacterial pages. A new gram staining image of S. enterica has also been included in VetBact.

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New field heading in VetBact

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Cultivation of E. coli on BG agar, which is used to differentiate between E. coli and salmonellas.

To avoid getting too many images under the heading “Macromorphology” in VetBact, we have introduced the new heading “Special media” (see e.g. Escherichia coli). Our intention is to use this new heading for images of bacterial colonies cultivated on special media used for isolation and/or identification of the  bacterium in question and not for many other bacteria. Under the heading Macromorphology, we will present pictures of bacterial colonies cultivated on “standard media” (e.g. blood agar, purple agar and FA agar). It will take some time to transfer images to this new heading.

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More than 300 images at VetBact

Over 300 pictures are included in VetBact (you can see thumb nail images of most of them by chosing  Images on the start page). In connection with the latest course in Bacteriology for veterinary students (sep-oct 2011), several new pictures were added in VetBact, for instance:

  • Purple agar plates with maltose for Staphylococcus  aureus subsp. aureus, S. hyicus and S. pseudintermedius. The metod is important to differentiate S. aureus  subsp. aureus from S. pseudintermedius and S. intermedius. All three species are positive for maltose in tube fermentation, but on agar plates, where the conditions are more aerobic, S. pseudintermedius and S. intermedius are negative (or possibly weakly positive).
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Colonies of Klebsiella pneumoniae subsp. pneumoniae cultivated on CLED agar.

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The first case of a Brucella canis infection of a dog in Sweden

Karl-Erik Johansson (BVF, SLU) is preparing agar plates with Brucella canis for photography in the safety lab at SVA . Photo: Lennart Melin (SVA).

Samples from a bitch which gave birth to stillborn pups were analyzed during the summer 2011 at the Department of Bacteriology of the National Veterinary Institute (SVA) in Uppsala. Brucellosis caused by Brucella canis was suspected and  the isolate was, therefore, sent to the EU Reference Laboratory for Brucellosis in France for further characterization, where Brucella canis was confirmed.

The bitch had been imported from Poland, where it was mated  with different male dogs that were suspected of having infected the bitch. Brucellosis is a serious disease, which can be transmitted to humans (a so-called zoonosis) and the infected dog was euthanized to prevent further transmission.

Pictures of the strain of B. canis that caused the first Swedish case of brucellosis on a dog can be found on the page for Brucella canis at VetBact.

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Image Quiz at VetBact

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Do you recognize this bacterium from its very typical appearence after Gram staining? If not, try the Image Quiz!

Different types of quizzes were added to VetBact during the last year. When the bacteriology course for veterinary students started this autumn, a new kind of quiz – the Image Quiz (IQuiz) – was released. In the IQuiz, you get a very brief description of a case and some images of the bacterium. From these data, it may be possible to identify the bacterium. If not, you can ask for more pictures and also hints.

You can find all quizzes under “Bacteriology Course 2011” on the start page of VetBact. Then, click on “Image quiz” to check this new type of quiz. Try also the “Disease quiz“, where you will get a continuous compilation of your results. Good Luck!

The other new module of VetBact – the VetBactLab – for experiments in silico, is under evaluation and has not yet been released without a password.

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Welcome to the Bacteriology course, 2011!

The bacteriology course for the veterinary students, 2008.

On September 20 the bacteriology course starts for the veterinary students. We hope that  VetBact will be useful during your studies and we appreciate all kinds of feed-back, which will help us to improve VetBact and its modules. One of the new modules in VetBact is VetBactLab that can be used for “experiments in silico” (dry experiments) and which we hope you will find interesting during your studies and later in your profession.

Feed-back can be given in different ways:

  1. By leaving a comment on this post (in English or Swedish) in the VetBactBlog. The comment will be published on the Blog if it is relevant.
  2. By using the contact form, which can be reached from most pages of VetBact (particularly useful if you want to comment or ask a question about a certain bacterial species).
  3. By leaving a comment on the form, which can be opened when you have finished a case in VetBactLab (particularly useful if you want to comment on the in silico cases).

The VetBactRef group

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